Journal: Advanced Science

Article Title: Lipid Droplet‐Derived Biomimetic Nanocarriers for the Enhancement of Porcine Intermuscular Fat Content

doi: 10.1002/advs.202406150

Figure Lengend Snippet: OA‐aLDs increased IMF in longissimus dorsi of Porcine. A. Schematic diagram of the pig injection test. Twelve 100‐day‐old healthy male Tongcheng pigs were used for the experiment. B) Measurement of IMF content in the longest dorsal muscle. * p < 0.05. C) Above: Image of the cross‐section of the longest dorsal muscle after injection. Arrows indicate intramuscular fat deposits. Relative area of adipose was counted by ImageJ, n = 3, * p < 0.05. Below: Pork separated longitudinally into two parts along the midline of the spine. Left side, NC; right side, injection. D) Measurement of carcass and meat quality traits. * p < 0.05. E) Oil red O staining of the longest dorsal muscle. Scale bar: 100 µm. n = 3, * p < 0.05. F) Confocal images of tissue fluorescence staining in longissimus dorsi. Scale bar: 100 µm. n = 3, * p < 0.05. G) Statistical plot of the relative percentage of multiple fatty acid components. * p < 0.05. H) Statistical chart of the proportion of saturated, monounsaturated, and polyunsaturated fatty acids. * p < 0.05. I) Elisa assay of Porcine TNF‐α and IL‐1β content. * p < 0.05. K) Relative mRNA expression of genes related to lipid metabolism in longissimus dorsi after injected OA‐aLDs. * p < 0.05. L) Detection of genes and pathways related to lipid synthesis after injected with OA‐aLDs in gastrocnemius muscle by Western Blot.

Article Snippet: Mouse IL‐6 Uncoated ELISA (88‐7064), Mouse TNF alpha Uncoated ELISA (88‐7324), Mouse IL‐1 beta Uncoated ELISA (88‐7013), Mouse IL‐10 Uncoated ELISA (88‐7105), Mouse C‐Reactive Protein/CRP ELISA Kit (EK294/2‐48, Liankebio), Porcine TNF‐alpha ELISA Kit (ES24RB, Thermofisher) and Porcine IL‐1 beta ELISA Kit (ESIL1B, Thermofisher) were used for ELISA detection.

Techniques: Injection, Staining, Fluorescence, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot